Areas of Interest:
Structure-function relationship investigations in amylolytic enzymes and other carbohydrases. This comprises: site-directed mutagenesis and kinetic and stability characterization of mutant proteins in combination with limited proteolysis to produce functional domains, protein crystallization, X-ray crystallography (in external collaboration), proteinaceous amylase inhibitor - amylase interaction studies by chemical, kinetic (pre steady state kinetics in external collaboration), mutational and crystallographic techniques. Primary structure comparison among amylolytic and related enzymes. B & AB Editor's Interests Protein engineering; mutational analysis; structure-function relationship investigations in enzymes and proteins. Carbohydrases; enzyme mechanism; specificity; protein structure; two- and three-dimensional; domain functions. alpha-Amylase structural family; sequence comparison. Enzyme (carbohydrase)-substrate/analogue interaction; mutational analysis of structure/function relationships; heterologous production of proteins; covalent structure of recombinant proteins; protein purification and characterization. Carbohydrases; protein engineering; amylolytic enzymes; plant proteins (cereals); structure-function relationships in proteins.
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